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(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)

Cultures can be maintained by addition of fresh medium, or by collecting cells as a pellet after centrifugation. The packed cells are then resuspended in fresh medium. The initial inoculum should not be less than 2 to 3 x 10⁵ viable cells/mL. A maximum of 1 to 2 x 10⁶ cells/mL are obtainable. Do not aspirate suspension to break up clumped cells.

Yosida TH, et al. Chromosomal alteration and development of tumors. XIX. Chromosome constitution of tumor cells in 16 plasma cell neoplasms of BALB/c mice. J. Natl. Cancer Inst. 41: 1083-1097, 1968. PubMed: 4176942

Potter M, Robertson CL. Development of plasma-cell neoplasms in BALB/c mice after intraperitoneal injection of paraffin-oil adjuvant, heart-killed Staphylococcus mixtures. J. Natl. Cancer Inst. 25: 847-861, 1960. PubMed: 13737512

Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.