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MECA-79
MECA-79
規(guī)格:
貨期:
編號:B165140
品牌:Mingzhoubio

標準菌株
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DNA
RNA

規(guī)格:
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產(chǎn)品名稱 MECA-79
商品貨號 B165140
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyer's patches.
It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV).
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin)
Comments
Animals were immunized with mouse lymph node stroma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyer's patches.
It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV).
It inhibits L-selectin dependent lymphocyte homing to lymph nodes and blocks lymphocyte binding to HEV in vitro.
This line grows best when cultured with a feeder layer of mouse (BALB/c) peritoneal exudate cells.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%; supplemented with 0.05 mM
2-mercaptoethanol. Note: A feeder layer of mouse peritoneal exudate or
irradiated murine fibroblasts (e.g. irradiated STO cells, ATCC catalog # X-56)
is required for recovery and growth of the MECA-79.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Culture Conditions
Temperature: 37.0°C
Isotype IgM
Name of Depositor Stanford University Board of Trustees
Deposited As rat (B cell); mouse (myeloma)
U.S. Patent Number
References

Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995

Berg EL, et al. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J. Cell Biol. 114: 343-349, 1991. PubMed: 1712790

Berg EL, et al. L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature 366: 695-698, 1993. PubMed: 7505053

Hemmerich S, et al. Sulfation-dependent recognition of high endothelial venules (HEV)- ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J. Exp. Med. 180: 2219-2226, 1994. PubMed: 7525849

Michie SA, et al. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am. J. Pathol. 143: 1688-1698, 1993. PubMed: 8256856

. . Cell. Biol. 107: 1853-1862, 1988.

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