| 產(chǎn)品名稱 |
Human adenovirus D |
| 商品貨號 |
B233612 |
| Classification |
Adenoviridae, Mastadenovirus |
| Agent |
Human adenovirus D |
| Strain |
A.A. |
| Common Name |
Human adenovirus 13 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
?-70°C or colder |
| Comments |
Recognized Reference Strain: Pereira HG, et al.A short description of adenovirus group. Virology 20: 613, 1963. Agglutinates both rat and rhesus RBC but usually the former to a higher titer than the latter. Contains soluble group-specific complement fixing antigen. |
| Effect on Host |
CPE, rounding and sloughing |
| Recommended Host |
KB cells (ATCC® CCL-17™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are 60-90% confluent. Remove medium and inoculate with a small volume (e.g. 1mL per 25 cm squared) of virus diluted with VGM to provide a MOI of about 0.1-1.0. Adsorb 1-2 hours at 37°C in a 5% CO2 atmosphere. End adsorbtion by adding VGM.
Incubation: Incubate infected culture for 1-6 days at 37°C in a 5% CO2 humidified atmosphere until CPE is well advanced through 90% of culture. |
| Name of Depositor |
RJ Huebner, NIH |
| Chain of Custody |
Deposited as Adenovirus type 13 |
| Source |
Anal swab from healthy child, Washington, DC, 1955 |
| References |
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
Rowe WP, et al. Serotype composition of the adenovirus group. Proc. Soc. Exp. Biol. Med. 97: 465-470, 1958. PubMed: 13518306
|
