亚洲国产精品一区二区久久hs,精品国产一级在线观看,免 费 成人黄 色 大片,不卡中文一二三区,国产剧情麻豆mv在线观看,久久久久久久久久久精品,久久九九综合,久久久久久91香蕉国产

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > IgH-2
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
IgH-2
IgH-2
規(guī)格:
貨期:
編號(hào):B164848
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 IgH-2
商品貨號(hào) B164848
Organism iguana
Tissue heart
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age immature
Gender male
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 35; range = 30 to 64.
The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere.
Virus Susceptibility iguana
Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Human poliovirus 1
Vesicular stomatitis virus
Complete Growth Medium Eagles's Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 36°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension into new culture vessels.
  6. Incubate cultures at 36°C in a closed flask without CO2 in air atmosphere.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 36°C
Atmosphere: Air, 100%; no CO2
Name of Depositor HF Clark
Deposited As iguana
Year of Origin August, 1966
References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Cross References

Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3).

Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4).

Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene.

Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene.

Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
克拉玛依市| 潜江市| 高雄市| 海口市| 兴安盟| 集安市| 富平县| 乐平市| 乌兰浩特市| 舞阳县| 蓬溪县| 潮州市| 晋中市| 张家界市| 井陉县| 和顺县| 拜泉县| 广德县| 会东县| 甘泉县| 神农架林区| 盐源县| 奉化市| 淅川县| 江川县| 奈曼旗| 巴彦县| 漳平市| 吴川市| 蛟河市| 阳朔县| 大余县| 宁化县| 呼玛县| 襄樊市| 台北市| 杭锦后旗| 铁岭市| 扬州市| 新密市| 天水市|