| 產(chǎn)品名稱 |
HT-144 |
| 商品貨號(hào) |
B164800 |
| Organism |
Homo sapiens, human |
| Tissue |
malignant melanoma; derived from metastatic site: subcutaneous tissue |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
malignant melanoma |
| Age |
29 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
(P15) hypotriploid to hypertriploid (+A, +B, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, minutes, secondary constrictions, breaks, large submetacentric, subtelocentric and minute markers |
| Derivation |
This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh. |
| Clinical Data |
29 years Caucasian male |
| Antigen Expression |
Blood Type O; Rh+; HLA A1, Aw24, B13, B15, Cw3, DRw4, DRw7 |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms anaplastic malignant tumor consistent with melanoma; tumors also form in steroid treated hamsters |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 12 D13S317: 11,12 D16S539: 12,13 D5S818: 11,13 D7S820: 11 THO1: 6,9 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes |
AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
J Fogh |
| Deposited As |
Homo sapiens |
| References |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887
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