| 產(chǎn)品名稱 |
G-402 |
| 商品貨號(hào) |
B164484 |
| Organism |
Homo sapiens, human |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial-like |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
renal leiomyoblastoma |
| Age |
9 months |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is a human cell line with the hyperdiploid chromosome count. The modal chromosome number was 47, occurring in 40% of cells. The rate of higher ploidies was 0.3%. The karyotype was 47,XX,+2,-5,+?ins(4) (q11.2) for the modal cell population. No other karyotypic abnormalities were evident. |
| Clinical Data |
9 months Caucasian female |
| Comments |
The line will grow in soft agar. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
PT Peebles |
| Deposited As |
Homo sapiens |
