| 產(chǎn)品名稱(chēng) |
CCRF S-180 II |
| 商品貨號(hào) |
B164216 |
| Organism |
Mus musculus, mouse |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
sarcoma |
| Age |
adult |
| Strain |
CFW |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Tumorigenic |
Yes |
| Effects |
Yes, in CFW mice |
| Virus Resistance |
poliovirus 1 |
| Comments |
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 95%; bovine calf serum, 5%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and add fresh medium. Aspirate cells and dispense into new flasks. |
| Name of Depositor |
GE Foley |
| Deposited As |
Mus musculus |
| References |
Foley GE, et al. Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines. Cancer Res. 20: 930-939, 1960. PubMed: 13823721
Foley EG, Drolet BP. Sustained propagation of sarcoma of 180 in tissue culture. Proc. Soc. Exp. Biol. Med. 92: 347-352, 1956. PubMed: 13350343
|
