亚洲国产精品一区二区久久hs,精品国产一级在线观看,免 费 成人黄 色 大片,不卡中文一二三区,国产剧情麻豆mv在线观看,久久久久久久久久久精品,久久九九综合,久久久久久91香蕉国产

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > C7
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
C7
C7
規(guī)格:
貨期:
編號:B164100
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 C7
商品貨號 B164100
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with bovine and human LDL receptor, and can be used to affinity purify LDL receptors.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Mice were immunized with partially purified bovine adrenal LDL receptor.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor
Comments
Mice were immunized with partially purified bovine adrenal LDL receptor.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with bovine and human LDL receptor, and can be used to affinity purify LDL receptors.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 104 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor JL Goldstein
Deposited As mouse (B cell); mouse (myeloma)
References

Beisiegel U, et al. Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J. Biol. Chem. 256: 11923-11931, 1981. PubMed: 6271765

Schneider WJ, et al. Purification of the low density lipoprotein receptor, an acidic glycoprotein of 164,000 molecular weight. J. Biol. Chem. 257: 2664-2673, 1982. PubMed: 6277909

Beisiegel U, et al. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J. Biol. Chem. 257: 13150-13156, 1982. PubMed: 6290495

Tolleshaug H, et al. Post translational processing of the LDL receptor and its genetic disruption in familial hypercholesterolemia. Cell 30: 715-724, 1982. PubMed: 6291781

Tolleshaug H, et al. The LDL receptor locus in familial hypercholesterolemia: multiple mutations disrupt transport and processing of a membrane receptor. Cell 32: 941-951, 1983. PubMed: 6299582

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
巴彦淖尔市| 苗栗市| 辰溪县| 彭州市| 交口县| 额敏县| 苏尼特左旗| 凤冈县| 安龙县| 四子王旗| 安多县| 隆昌县| 渝北区| 东兴市| 剑河县| 扎鲁特旗| 偏关县| 枞阳县| 伽师县| 南涧| 西昌市| 灵台县| 浮梁县| 永修县| 通许县| 保康县| 肥乡县| 诸城市| 板桥市| 石楼县| 昌图县| 措美县| 濮阳市| 辽阳市| 丰都县| 漠河县| 河南省| 南漳县| 海阳市| 宁阳县| 甘南县|