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C305
C305
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貨期:
編號:B164082
品牌:Mingzhoubio

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產(chǎn)品名稱 C305
商品貨號 B164082
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152). Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells. It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Genes Expressed
immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Cellular Products
immunoglobulin; monoclonal antibody; against the beta chain of the T cell antigen receptor on Jurkat cells
Comments
Animals were immunized with cells from the human T cell line, Jurkat, E6-1 (ATCC TIB-152). Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for the beta chain of the T cell antigen receptor (TCR) on Jurkat cells. It is useful for studying the T cell receptor signaling pathway using the Jurkat cell model.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension between 1 x 105  and 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases).
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgM kappa
Name of Depositor A Weiss
Deposited As mouse (B cell); mouse (myeloma)
References

Weiss A, Stobo JD. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J. Exp. Med. 160: 1284-1299, 1984. PubMed: 6208306

Goldsmith MA, Weiss A. Early signal transduction by the antigen receptor without commitment to T cell activation. Science 240: 1029-1031, 1988. PubMed: 3259335

Wu J, et al. Vav and SLP-76 interact and functionally cooperate in IL-2 gene activation. Immunity 4: 593-602, 1996. PubMed: 8673706

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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